Type III Secretion Lab
Carmen R. Beuzón & Javier Ruiz-Albert
Department of Plant-Microorganism-Insect Interaction
Institute of Subtropical and Mediterranean Horticulture (IHSM)
University of Malaga-Higher Council for Scientific Research (UMA-CSIC)
Our research focuses on the molecular interaction between the plant and the phytopathogenic bacteria Pseudomonas syringae, a study model for its academic and economic relevance, and using as experimental pathosystems P. syringae pv tomato/ Arabidopsis and P. syringae pv. phasoelicola/ bean. The central axis of this research is the type III secretion system and its effectors, which we study from the regulation of its expression, with special emphasis on its role in the generation of functionally different subpopulations in populations of the pathogen, at its functional characterization, including the molecular mechanisms by which effectors suppress plant defenses (PTI, ETI, SAR), the effect on their eukaryotic targets and impact on the processes in which they participate, and the cooperation between effectors. Recently, we are applying the knowledge generated to characterize the mechanisms by which the human pathogen Salmonella enterica colonizes tomato.
https://www.ihsm.uma-csic.es/investigadores/16 https://www.ihsm.uma-csic.es/investigadores/28
Members:
Jose S. Rufián Plaza
Nieves López Pagán
Javier Rueda Blanco
Angel del Espino Pérez
Laura Mancera Miranda
Publications
Zarkani AA, López-Pagán N, Grimm M, Sánchez-Romero MA, Ruiz-Albert J, Beuzón CR Schikora A (2020) Salmonella Heterogeneously Expresses Flagellin during Colonization of Plants. Microorganisms 8:815 doi:10.3390/microorganisms8060815
Arce-Leal AP, Bautista R, Rodríguez-Negrete EA, Manzanilla-Ramírez MA, Velázquez-Monreal JJ, Santos-Cervantes ME, Méndez-Lozano J, Beuzón CR, Bejarano ER, Castillo AG, Claros MG, Leyva-López NE ( 2020) Gene expression profile of Mexican lime trees in response to inoculation with Candidates Liberibacter asiaticns. Microorganisms 7; 8 (4). pii: E528. doi: 10.3390 / microorganisms8040528.
López-Márquez D, Del-Espino Ángel, Bejarano ER, Beuzón CR*, Ruiz-Albert J* (2020) Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis. En prensa in Plant Methods. doi: 10.1186/s13007-020-00581-PLME-D-19-00303R2.
Rufián JS, Rueda-Blanco J, Beuzón CR*, Ruiz-Albert J* (2019) Protocol: an improved method to quantify activation of systemic acquired resistance (SAR). Plant Methods 15:16. doi: 10.1186/ s13007-019-0400-5).
Rufián JS, Lucía A, Rueda-Blanco J, Zumaquero A, Guevara CM, Ortíz-Martín I, Ruiz-Aldea G, Macho AP, Beuzón CR *, Ruiz-Albert J * (2018) Suppression of HopZ effector-triggered plant immunity in a natural pathosystem. Frontiers in Plant Science 14:977. doi: 10.3389/ fpls.2018.00977).
Rufián JS, Macho AP, Corry DS, Mansfield JW, Ruiz-Albert J, Arnold DL, Beuzón CR* (2018) Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains. Molecular Plant Pathology. doi: 10.1111/ mpp.12439
Charova SN, Gazi AD, Mylonas E, Pozidis C, Sabarit B, Anagnostou D, Psatha K, Aivaliotis M, Beuzon CR, Panopoulos NJ, Kokkinidis M (2018) Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion. mBio. doi: 10.1128 / mBio.01096-18
Rufián JS, López-Márquez D, López-Pagán N, Grant M, Ruiz-Albert J, Beuzón CR * (2018) Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae. Methods Mol Biol. 1734:183-199. doi: 10.1007/978-1-4939-7604-1_15.
Castro PH, Santos MÂ, Freitas S, Cana-Quijada P, Lourenço T, Rodrigues MAA, Fonseca F, Ruiz-Albert J, Azevedo JE, Tavares RM, Castillo AG, Bejarano ER, Azevedo H. (2018) Arabidopsis thaliana SPF1 and SPF2 are nuclear-located ULP2-like SUMO proteases that act downstream of SIZ1 in plant development. J Exp Bot. 69: 4633-4649. doi: 10.1093 / jxb / ery265
Arroyo-Mateos M, Sabarit B, Maio F, Sánchez-Durán MA, Rosas-Díaz T, Prins M, Ruiz-Albert J, Luna AP, van den Burg HA, Bejarano ER. (2018) Geminivirus Replication Protein Impairs SUMO Conjugation of Proliferating Cellular Nuclear Antigen at Two Acceptor Sites. J Virol. 92:e00611-18. doi: 10.1128/JVI.00611-18.
Rufián JS, Sánchez-Romero M-A, López-Márquez D, Macho AP, Mansfield JW, Arnold DL, Ruiz-Albert J, Casadesús J, Beuzón CR* (2016) Pseudomonas syringae differentiates into phenotypically distinct subpopulations during colonization of a plant host. Environmental microbiology. doi: 10.1111/ 1462-2920.13497.
Calvo-Polanco M, Sánchez-Castro I, Cantos M, García JL, Azcón R, Ruiz-Lozano JM, Beuzón CR, Aroca R (2016) Effects of different arbuscular mycorrhizal fungal backgrounds and soils on olive plants growth and water relation properties under well-watered and drought conditions. Plant Cell Environ. 39:2498-2514. doi: 10.1111/pce.12807.
Rosas-Díaz T, Macho AP, Beuzón CR, Lozano-Durán R, Bejarano ER. (2016) The C2 Protein from the Geminivirus Tomato Yellow Leaf Curl Sardinia Virus Decreases Sensitivity to Jasmonates and Suppresses Jasmonate-Mediated Defences. Plants 15;5(1). pii: E8. doi: 10.3390/plants5010008.
Aussel L, Beuzón CR, Cascales E (2016) Meeting report: Adaptation and communication of bacterial pathogens. Virulence. 18:481-90. doi: 10.1080/21505594.2016.1152441.
Macho AP, Rufián JS, Ruiz-Albert J, Beuzón CR * (2016) Competitive Index: Mixed Infection-Based Virulence Assays for Genetic Analysis in Pseudomonas syringae-Plant Interactions. Methods Mol Biol. 1363:209-17. doi: 10.1007/978-1-4939-3115-6_17.
Castro PH, Couto D, Freitas S, Verde N, Male AP, Huguet S, Botella MA, Ruiz-Albert J, Tavares RM, Bejarano ER, Azevedo H. (2016) SUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thaliana. Plant Mol Biol. 92:143-59. doi: 10.1007/s11103-016-0500-9.
Rufián JS, Lucía A, Macho AP, Orozco-Navarrete B, Arroyo-Mateos MA, Bejarano ER, Beuzón CR*, Ruiz-Albert J* (2015) Auto-acetylation on K289R is not essential for HopZ1a-mediated plant defence suppression. Frontiers in Microbiology. doi: 10.3389 / fmicb.2015.00684.